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First evidence of predation on the native endangered Galemys pyrenaicus by the invader specie American mink using eDNA tools in Extremadura (Spain)
Год: 2024
Авторы: Ripa A., Palacios-Gonzalez M.J., Díaz-Caballero J.A., Espinosa A., Zalba F.J., García-Zapata J.L., Fernádez-García J.L.,
Издание: preprint on Research Square
In recent years it has been claimed that the introduction of invasive species significantly damages ecosystems. Their ability to adapt has been overestimated, without considering the consequences they could have on the native fauna. One of the clearest examples is the American mink (Neovison vision), which has also been postulated as a large predator. Its diet includes small native mammals such Mediterranean water shrew (Neomys anomalus), also one of great importance, Iberian desman (Galemys pyrenaicus), which today is seriously endangered due to the population decline it has suffered in recent years. There are very few studies that have been carried out in the Central System Mountains of the region of Extremadura that help to understand the impact of the diet of these invasive species. Thanks to non-invasive sampling through faecal collection and non-invasive genetic techniques, we can track the impact of predators such as mink and. The assay was developed using the TaqMan probe design for duplex RT-PCR simultaneously targeting the CYB genes of the native species G. pyrenaicus and Neomys anomalus, demonstrating the evidence of the presence of micromammals. Therefore, the assay has high analytical sensitivity for both target species, and is a tool that will helps to monitor the damage of invasive species. Faeces, Galemys pyrenaicus, Identification, invasive species, Neovison vison, Noninvasive samples, rt-PCR
https://doi.org/10.21203/rs.3.rs-3923939/v1
Тэги: Faeces, Galemys pyrenaicus, Identification, invasive species, Neovison vison, Noninvasive samples, rt-PCR
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